The main objective of the present research is to develop questionnaire items in Japanese language learning for Indonesian university students. A total of 157 university students participated in the study and completed the questionnaire online. First, an exploratory factor analysis was conducted in order to investigate a structure of motivational factors, which extracted a six-factor structure. Confirmatory factor analysis was then conducted through structural equation modeling and the validity of the six-factor model was examined. The Cronbach alpha coefficients were also calculated. The results of the two factor analyses provided a satisfactory reliability and validation of the questionnaire. Consequently, the extracted six-factor motivational structure was certified as an appropriate questionnaire battery to assess Japanese language learning motivation in Indonesia university students.