The purpose of this research was to investigate the experiences of experiment and observation, and domestication of living things in the secondary school biology. The questionnaire item were as followed: the number of experiences about experiment and observation in the secondary school biology, the number of experiences using microscope in the school, the number of experiences in field activity, the experience and the name of variety of living things for domestication and so on. The result of the analysis, the average number of experience about experiment and observation was once a month in the junior high school, and several times a year in the high school biology. The experiment and observation about "Cell" and "Metabolism" in the high school biology, and that about "Plant" in the junior high school were more experienced than the other units. The average number of the microscope use was within 5 times in any school stage. The experiences of field activity, animal keeping and plant cultivation were few chances in the secondary schools. Especially the experiences of domestication of living things were originated to the elementary schools.