この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00038292
Hiroshima Journal of Medical Sciences 33 巻 4 号
1984-12 発行
Attempt at Use of the Fluorometric Neuraminidase Assay System for the Enzyme Antibody Inhibition Test
ノイラミニダーゼ活性の螢光比色法を用いるノイラミニダーゼ阻止試験の試み
Kiyotani Katsuhiro
Takei Naomi
Matsuo Yoshiyasu
Abstract
The fluorometric neuraminidase assay method using 4-methylumbelliferyl-N-Ac-α-D-neuraminide as a substrate was investigated for the purpose of ascertaining its validity as a procedure for influenza viral neuraminidase antibody inhibition test.
The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidase assay method using fetuin as a substrate. Although solubilization of viral neuraminidase spikes with Triton X-100 increased the enzyme inhibition of antiserum to some extent, there is little possibility of using the fluorometric assay system for the purpose.
The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidase assay method using fetuin as a substrate. Although solubilization of viral neuraminidase spikes with Triton X-100 increased the enzyme inhibition of antiserum to some extent, there is little possibility of using the fluorometric assay system for the purpose.
About This Article
著者キーワード
Neuraminidase
Neuraminidase inhibition test
Influenza virus 4-Methylumbelliferyl-N- Ac-α-D-neuraminide
Other Article
PP. 571 - 575
PP. 585 - 589
PP. 591 - 594
PP. 595 - 598
PP. 607 - 614
PP. 615 - 618
PP. 619 - 624
PP. 649 - 657
PP. 659 - 662
PP. 679 - 683
PP. 715 - 720
PP. 727 - 730
PP. 747 - 754
PP. 755 - 758
PP. 759 - 763
PP. 771 - 774
PP. 813 - 817