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Hiroshima Journal of Medical Sciences Volume 33 Issue 4
published_at 1984-12
Attempt at Use of the Fluorometric Neuraminidase Assay System for the Enzyme Antibody Inhibition Test
ノイラミニダーゼ活性の螢光比色法を用いるノイラミニダーゼ阻止試験の試み
Kiyotani Katsuhiro
Takei Naomi
Matsuo Yoshiyasu
Abstract
The fluorometric neuraminidase assay method using 4-methylumbelliferyl-N-Ac-α-D-neuraminide as a substrate was investigated for the purpose of ascertaining its validity as a procedure for influenza viral neuraminidase antibody inhibition test.
The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidase assay method using fetuin as a substrate. Although solubilization of viral neuraminidase spikes with Triton X-100 increased the enzyme inhibition of antiserum to some extent, there is little possibility of using the fluorometric assay system for the purpose.
The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidase assay method using fetuin as a substrate. Although solubilization of viral neuraminidase spikes with Triton X-100 increased the enzyme inhibition of antiserum to some extent, there is little possibility of using the fluorometric assay system for the purpose.
About This Article
Keywords
Neuraminidase
Neuraminidase inhibition test
Influenza virus 4-Methylumbelliferyl-N- Ac-α-D-neuraminide
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