Attempt at Use of the Fluorometric Neuraminidase Assay System for the Enzyme Antibody Inhibition Test
Hiroshima Journal of Medical Sciences 33 巻 4 号
825-827 頁
1984-12 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Attempt at Use of the Fluorometric Neuraminidase Assay System for the Enzyme Antibody Inhibition Test
|
| タイトル ( jpn ) |
ノイラミニダーゼ活性の螢光比色法を用いるノイラミニダーゼ阻止試験の試み
|
| 作成者 |
Kiyotani Katsuhiro
Takei Naomi
Matsuo Yoshiyasu
|
| 収録物名 |
Hiroshima Journal of Medical Sciences
|
| 巻 | 33 |
| 号 | 4 |
| 開始ページ | 825 |
| 終了ページ | 827 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
The fluorometric neuraminidase assay method using 4-methylumbelliferyl-N-Ac-α-D-neuraminide as a substrate was investigated for the purpose of ascertaining its validity as a procedure for influenza viral neuraminidase antibody inhibition test.
The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidase assay method using fetuin as a substrate. Although solubilization of viral neuraminidase spikes with Triton X-100 increased the enzyme inhibition of antiserum to some extent, there is little possibility of using the fluorometric assay system for the purpose. |
| 著者キーワード |
Neuraminidase
Neuraminidase inhibition test
Influenza virus 4-Methylumbelliferyl-N- Ac-α-D-neuraminide
|
| NDC分類 |
医学 [ 490 ]
|
| 言語 |
英語
|
| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University School of Medicine
|
| 発行日 | 1984-12 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6398818
|
