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Hiroshima Journal of Medical Sciences Volume 33 Issue 4
published_at 1984-12
Establishment and Characterization of New Cell Line (YMB-1) Derived from Human Breast Carcinoma
ヒト乳癌細胞株（YMB-1）の樹立とその特性
Yamane Motoi
Nishiki Masayuki
Kishi Naohiko
Amano Kuniki
Nakagawa Kenichi
Okumichi Tsuneo
Naito Masashi
Ito Akihiko
Ezaki Haruo
Abstract
A continuous cell line (YMB-1), derived from a primary breast cancer lesion has been established and maintained in culture for the past 9 months. Cell colonies consist of polygonal epithelial cells containing junctional complexes and tubular formation. The cell population doubling time is 44 hr and the saturation density at confluency is 2.5×10^5 cells/cm2. The chromosome modal number is 73 and 3 marker chromosomes are visible. Both cytosol estrogen and androgen receptors are present. However, the cytosol progesterone receptor was not detected by the dextran coated chacoal method. The s. c. injection of 5×10^6 cells from cell culuture into nude mice induced a tumor.
Both the spillage method and cell separation by specific gravity were employed to remove fibroblasts.
Both the spillage method and cell separation by specific gravity were employed to remove fibroblasts.
About This Article
Keywords
Established cell line
Human breast cancer
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