Establishment and Characterization of New Cell Line (YMB-1) Derived from Human Breast Carcinoma

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タイトル ( eng )
Establishment and Characterization of New Cell Line (YMB-1) Derived from Human Breast Carcinoma
タイトル ( jpn )
ヒト乳癌細胞株(YMB-1)の樹立とその特性
作成者
Yamane Motoi
Nishiki Masayuki
Kishi Naohiko
Amano Kuniki
Nakagawa Kenichi
Okumichi Tsuneo
Naito Masashi
Ito Akihiko
Ezaki Haruo
収録物名
Hiroshima Journal of Medical Sciences
33
4
開始ページ 715
終了ページ 720
収録物識別子
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
抄録
A continuous cell line (YMB-1), derived from a primary breast cancer lesion has been established and maintained in culture for the past 9 months. Cell colonies consist of polygonal epithelial cells containing junctional complexes and tubular formation. The cell population doubling time is 44 hr and the saturation density at confluency is 2.5×10^5 cells/cm2. The chromosome modal number is 73 and 3 marker chromosomes are visible. Both cytosol estrogen and androgen receptors are present. However, the cytosol progesterone receptor was not detected by the dextran coated chacoal method. The s. c. injection of 5×10^6 cells from cell culuture into nude mice induced a tumor.

Both the spillage method and cell separation by specific gravity were employed to remove fibroblasts.
著者キーワード
Established cell line
Human breast cancer
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 紀要論文
出版者
Hiroshima University School of Medicine
発行日 1984-12
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6534930