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Hiroshima Journal of Medical Sciences Volume 33 Issue 2
published_at 1984-06

Modification of the Fluorescent Staining　Method for Mycobacterial Cells

抗酸菌螢光染色法の改良
Tsukiyama Fumiaki
Katoh Masafumi
Matsuo Yoshiyasu
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HiroshimaJMedSci_33_293.pdf
Abstract
The fluorescein diacetate-ethidium bromide (FDA/EB) staining method for determining the viability of mycobacterial cells reported by Kvach and Veras was modified for applying the method to Mycobacterium leprae and Mycobacterium lepraemurium. Our method clearly differentiated green-stained cells from red-orange ones with either mycobacterial species and permitted their original fluorescence for over 4 min to be maintained.
About This Article
Keywords
FDA/EB staining
Mycobacterium leprae
Mycobacterium lepraemurium
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