この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00038214
Hiroshima Journal of Medical Sciences 33 巻 2 号
1984-06 発行
Firefly Luciferase Assay for Adenosine Triphosphate in Cultured Cells
ルシフェラーゼ試薬を用いる培養細胞の ATP 測定法
Kiyotani Katsuhiro
Kanamoto Yasuo
Matsuo Yoshiyasu
Abstract
A method for determining intracellular content of adenosine triphosphate (ATP) in the cultured cells was investigated with a firefly luciferase reagent, and a micro assay method was established. The method minimized the size of both samples and luciferase reagent employed.
The per cell amount of intracellular ATP of HeLa 229 and McCoy cells was estimated to be 7.20×10-11 M arid 8.74×10-11 M, respectively. The method could be applied to a direct measurement of the ATP content in cultured cells grown in each well of a 96-well micro tissue culture plate.
The per cell amount of intracellular ATP of HeLa 229 and McCoy cells was estimated to be 7.20×10-11 M arid 8.74×10-11 M, respectively. The method could be applied to a direct measurement of the ATP content in cultured cells grown in each well of a 96-well micro tissue culture plate.
About This Article
著者キーワード
Firefly luciferase
ATP
HeLa 229 cells
McCoy cells
Other Article
PP. 113 - 123
PP. 145 - 149
PP. 173 - 177
PP. 211 - 221
PP. 223 - 231
PP. 233 - 238
PP. 259 - 263