Alloantisera (anti-donor and anti-recipient alloantiserum) were prepared by skin grafting followed four inoculations of 1×10^7 splenocytes.
These alloantisera were cytotoxic (×64) to target cells specifically.
Anti-recipient alloantiserum was effective on enhancing skin graft survival in mice as same as anti-donor alloantiserum, when administered on the operative day, second postoperative day and fourth postoperative day between 50-200 μl at one time.