The spatial pattern of histochemically stained normal muscle fibers and reinnervated muscle fibers is called mosaic pattern or type grouping, respectively only from appearance. In this study we classified the spatial distribution of rats' anterior tibial muscle fibers into homogeneous, clustered and random distribution by the distance method of Hopkins and Skellam and quantitatively expressed the spatial distribution of each muscle fiber type using our newly developed computer system. The results showed that normal red and white muscle fibers are distributed homogeneously or at random, whereas reinnervated muscle fibers are in a clustered pattern and the degree of clustered pattern becomes larger with the lapse of time after reinnervation. These results suggest the possibility of quantitatively indicating the degree of muscle fiber recovery after reinnervation.