Modification of the Fluorescent Staining Method for Mycobacterial Cells

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Title ( eng )
Modification of the Fluorescent Staining Method for Mycobacterial Cells
Title ( jpn )
抗酸菌螢光染色法の改良
Creator
Tsukiyama Fumiaki
Katoh Masafumi
Matsuo Yoshiyasu
Source Title
Hiroshima Journal of Medical Sciences
Volume 33
Issue 2
Start Page 293
End Page 295
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
The fluorescein diacetate-ethidium bromide (FDA/EB) staining method for determining the viability of mycobacterial cells reported by Kvach and Veras was modified for applying the method to Mycobacterium leprae and Mycobacterium lepraemurium. Our method clearly differentiated green-stained cells from red-orange ones with either mycobacterial species and permitted their original fluorescence for over 4 min to be maintained.
Keywords
FDA/EB staining
Mycobacterium leprae
Mycobacterium lepraemurium
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University School of Medicine
Date of Issued 1984-06
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6384149