Modification of the Fluorescent Staining Method for Mycobacterial Cells
Hiroshima Journal of Medical Sciences Volume 33 Issue 2
Page 293-295
published_at 1984-06
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この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00038216
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fulltext
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Title ( eng ) |
Modification of the Fluorescent Staining Method for Mycobacterial Cells
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Title ( jpn ) |
抗酸菌螢光染色法の改良
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Creator |
Tsukiyama Fumiaki
Katoh Masafumi
Matsuo Yoshiyasu
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 33 |
Issue | 2 |
Start Page | 293 |
End Page | 295 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
The fluorescein diacetate-ethidium bromide (FDA/EB) staining method for determining the viability of mycobacterial cells reported by Kvach and Veras was modified for applying the method to Mycobacterium leprae and Mycobacterium lepraemurium. Our method clearly differentiated green-stained cells from red-orange ones with either mycobacterial species and permitted their original fluorescence for over 4 min to be maintained.
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Keywords |
FDA/EB staining
Mycobacterium leprae
Mycobacterium lepraemurium
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University School of Medicine
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Date of Issued | 1984-06 |
Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6384149
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