この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00049848
Hiroshima Journal of Medical Sciences Volume 34 Issue 1
published_at 1985-03
Partial Purification of Histaminase from Guinea-Pig Liver by Gel-Filtration at High Temperature
KOHRO Osamu
MORITA Eisin
YAMAMOTO Shoso
Abstract
Histaminase was partially purified by Sephadex G-200 gel filtration at high temperature. Guinea pig liver histaminase was extracted with heparin. The extract was fractionated by Sephadex G-200 gel filtration at 4°C. In the fractions containing histaminase, 7 dense and 2 faint protein bands were detected on SDS-gel electrophoresis. Further, fractionation of this sample by Sephadex G-200 gel filtration at 55°C markedly decreased the number of bands, i.e. only 1 dense and 2 faint bands were observed in the fractions in which histaminase activity was detected, and the enzyme activity was increased by approximately ten times. The results suggest that gel filtration at high temperature may be useful for partial purification of histaminase.
About This Article
Descriptions
This work was supported by Scientific Research Grant from the Japanese Ministry of Education (B, No. 58480264).
Keywords
Histaminase
Liver
Purification
Other Article
PP. 31 - 37
Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture
PP. 43 - 51
PP. 61 - 65
PP. 113 - 117
PP. 147 - 150
PP. 155 - 160