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Hiroshima Journal of Medical Sciences Volume 34 Issue 1
published_at 1985-03

Use of the Fluorescent Staining Method for Determining the Viability of Mycobacterium lepraemurium

TSUKIYAMA Fumiaki
KATOH Masafumi
NOMURA Toshiya
MATSUO Yoshiyasu
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HiroshimaJMedSci_34_161.pdf
Abstract
The cell suspension of Mycobacterium lepraemurium was exposed to heating of 40°C to 70°C for various lengths of time. The percent green-fluorescent cell by the midified fluorescein diacetate (FDA) -ethidium bromide (EB) staining method was calculated and compared with the infectivity to mice. The reduction in the percentage was associated significantly with the loss of the infectivity.
About This Article
Keywords
Mycobacterium lepraemurium
FDA/EB staining
Viability
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