Partial Purification of Histaminase from Guinea-Pig Liver by Gel-Filtration at High Temperature
Hiroshima Journal of Medical Sciences 34 巻 1 号
147-150 頁
1985-03 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Partial Purification of Histaminase from Guinea-Pig Liver by Gel-Filtration at High Temperature
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| 作成者 |
KOHRO Osamu
MORITA Eisin
YAMAMOTO Shoso
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| 収録物名 |
Hiroshima Journal of Medical Sciences
|
| 巻 | 34 |
| 号 | 1 |
| 開始ページ | 147 |
| 終了ページ | 150 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
Histaminase was partially purified by Sephadex G-200 gel filtration at high temperature. Guinea pig liver histaminase was extracted with heparin. The extract was fractionated by Sephadex G-200 gel filtration at 4°C. In the fractions containing histaminase, 7 dense and 2 faint protein bands were detected on SDS-gel electrophoresis. Further, fractionation of this sample by Sephadex G-200 gel filtration at 55°C markedly decreased the number of bands, i.e. only 1 dense and 2 faint bands were observed in the fractions in which histaminase activity was detected, and the enzyme activity was increased by approximately ten times. The results suggest that gel filtration at high temperature may be useful for partial purification of histaminase.
|
| 著者キーワード |
Histaminase
Liver
Purification
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| 内容記述 |
This work was supported by Scientific Research Grant from the Japanese Ministry of Education (B, No. 58480264).
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| NDC分類 |
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University School of Medicine
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| 発行日 | 1985-03 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3926723
|
