Partial Purification of Histaminase from Guinea-Pig Liver by Gel-Filtration at High Temperature
Hiroshima Journal of Medical Sciences Volume 34 Issue 1
Page 147-150
published_at 1985-03
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| File | |
| Title ( eng ) |
Partial Purification of Histaminase from Guinea-Pig Liver by Gel-Filtration at High Temperature
|
| Creator |
KOHRO Osamu
MORITA Eisin
YAMAMOTO Shoso
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 34 |
| Issue | 1 |
| Start Page | 147 |
| End Page | 150 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Histaminase was partially purified by Sephadex G-200 gel filtration at high temperature. Guinea pig liver histaminase was extracted with heparin. The extract was fractionated by Sephadex G-200 gel filtration at 4°C. In the fractions containing histaminase, 7 dense and 2 faint protein bands were detected on SDS-gel electrophoresis. Further, fractionation of this sample by Sephadex G-200 gel filtration at 55°C markedly decreased the number of bands, i.e. only 1 dense and 2 faint bands were observed in the fractions in which histaminase activity was detected, and the enzyme activity was increased by approximately ten times. The results suggest that gel filtration at high temperature may be useful for partial purification of histaminase.
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| Keywords |
Histaminase
Liver
Purification
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| Descriptions |
This work was supported by Scientific Research Grant from the Japanese Ministry of Education (B, No. 58480264).
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1985-03 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3926723
|
