この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00049832
Hiroshima Journal of Medical Sciences 34 巻 1 号
1985-03 発行
Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture
YAMAKIDO Michio
YANAGIDA Jitsuro
ISHIOKA Sinichi
MATSUZAKA Shigeru
HOZAWA Soichiro
AKIYAMA Mitoshi
KOBUKE Kyoko
INAMIZU Tsutomu
NISHIMOTO Yukio
Abstract
For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture.
1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5%
2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture.
3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS.
1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5%
2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture.
3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS.
About This Article
著者キーワード
Production of Interleukin 2
Human peripheral blood lymphocytes
Optimal condition
CTLL-2 microassay
Other Article
PP. 31 - 37
Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture
PP. 43 - 51
PP. 61 - 65
PP. 113 - 117
PP. 147 - 150
PP. 155 - 160