RNA POLYMERASES AND THEIR STIMULATING FACTOR FROM LEUKEMIA L1210 ASCITES CELLS

RNA polymerases I and II were partially purified from L1210 ascites cells by sonic disruption, batchwise treatment with diethylaminoethyl (DEAE) cellulose, ammonium sulfate-fractionation, and subsequent linear KCl gradient chromatograpy on DEAE cellulose column. The RNA polymerases from L1210 cells showed similar enzymatic properties as in cases of Ehrlich carcinoma-RNA polymerases. RNA polymerase-stimulating factor was obtained by carboxymethyl (CM) cellulose column chromatography from L1210 cell lysate. The factor enhanced both of RNA polymerases I and II when native calf thymus DNA was used as a template, although it failed to casuse any stimulating effect when poly dAT was employed as a template DNA.