Effect of FC43se on Endotoxin-induced Disseminated Intravascular Coagulation in Rats

Perfluorotributylamine/Pluronic F68 Stem-Emulsion (FC43se), which is a blood substitute, was assessed for its effectiveness on disseminated intravascular coagulation (DIC) in the rat model. Rats were infused intravenously with 2.5 mg/kg of Escherichia coli lipopolysaccharide (Escherichia coli 055:B5 lipopolysaccharide B) for four hours. At the same time, FC43se or normal physiological saline was infused at 2.5 ml/kg/hr. The white blood cell and platelet counts, prothrombin time (PT), activated partial thromboplastin time (APTT), and the plasma levels of interleukin-1 beta (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor alpha (TNFα) were determined at 4hr. The infusion of FC43se markedly prevented a decrease in platelet counts (p=0.0004) and a prolongation of both PT and APTT (p<0.05 and p<0.03 each). The serum level of IL-1βand IL-4 showed no significant change. The serum level of IL-6, IL-10 and TNFα increased significantly (p=0.0007, p=0.0004 and p<0.05 each) with infusion of FC43se in rats treated with bacterial endotoxin. FC43se has beneficial effects on endotoxin-induced DIC as an anticoagulant and anti-inflammatory cytokine induced agent.