Antitumor Effects of Natural-Human TNF on BDFI Mice Bearing Lewis Lung Carcinoma

Natural-human tumor necrosis factor (n-TNF) was obtained by isolating and refining lymphokines which were extracted from human acute lymphoblastic leukemia BALL-I cells. Antitumor effects of this n-TNF were studied by using Lewis lung carcinoma (3LL) which was transplanted on BDFI mice.
n-TNF showed inhibitory effects of the proliferation of metastatic tumors dose-dependently through i.v. injection daily for 10 days. And the study of the dose schedule of the administration and the route of the administration showed that routes of i.v., i.m. and i.t. injections were effective respectively through daily administration. Histological study showed effects which were ranked Grade Ilb (and partially III) of Shimosato and Ohboshi's histological criteria.