High Performance Gel-Chromatography as a Parameter for the Progress of Hepatic Failure in Dogs

Hepatic failure dogs induced by hepatic ischemia were prepared from mongrel dogs by occluding their hepatic and gastroduodenal arteries without anesthesia 48 hours after operation of their end to side port-ca val anastomosis. During the process, they were divided into two groups; the 60-minute temporary hepatic ischemia group and the continuous hepatic ischemia group.

The plasma was sampled with the lapse of time from the above dogs after their hepatic ischemia and was analyzed using a high performance gel-chromatography (TSK G2000SW). The progress of hepatic failure caused distinct increase in the 12 abnormal Peaks a-1, of which Peak a and b fell in a region of middle molecular weights on the chromatography. Especially, Peak b increased greatly showing a good correlation with the condition of progressing hepatic failure.

Therefore, it is considered that the measurement of variation of such peaks, especially of Peak b, by the high performance gel-chromatography will prove to be a parameter indicating the degree of hepatic failure in progress.