The isolation rate of Ureaplasma urealyticum in males was 45.3% in patients with urethritis, 43.9% with prostatitis, and 37.5% with cystitis, respectively. These values were significantly high compared with the rate of 28.8% in healthy control. The incidence of this organism in females was generally higher than that in males. It was 57.4% in patients with vaginitis, 47.8% with other genitourinary diseases, 51.6% in pregnant women, and 34.0% in healthy control. In prostitutes, the rate was as high as 77.6%. The isolation rate of Mycoplasma hominis was relatively low in both sexes, and no clear difference was observed between the patients and healthy controls. However, the rate was extremely high in prostitutes, amounting to 79.1%.