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Hiroshima Journal of Medical Sciences 36 巻 2 号
1987-06 発行

A Simple Method for the Observation of Cultured Rat Hepatocytes by Scanning Electron Microscopy

KASUGA Shigeo
TAGASHIRA Nobuharu
HARADA Yasuo
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6.79 MB
HiroshimaJMedSci_36_169.pdf
Abstract
A simple method for the three-dimensional observation of inner and outer ultrastructures of the cultured hepatocyte was presented. The freeze cracked plane of the cultured cell was obtained by means of cracking the underlying substrate. This method made it possible to observe the ultrastructural features of both cell surface and cytoplasm in the same cell. Among several substrates examined in this investigation, the collagen gel sheet was the most useful for this purpose.
About This Article
著者キーワード
Cultured hepatocytes
Scanning electron microscopy
Three-dimensional structure
Aldehyde-prefix osmium-DMSO-osmium method
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