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Hiroshima Journal of Medical Sciences Volume 31 Issue 2
published_at 1982-06-10

Effect of Filter on Specific (3H)-5-Hydroxytryptamine Binding to Synaptic Membranes

シナプス膜への特異的セロトニン結合に及ぼすフィルターの影響
Yamawaki Shigeto
Maeoka Kunihiko
Sarai Keisuke
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HiroshimaJMedSci_31_129.pdf
Abstract
In the present study, the authors carried out some basic examinations of specific (3H)-5-Hydroxytryptamine (5-HT, serotonin) receptor binding assay. The most adequate incubation condition for (3H)-5-HT binding assay was at 0°C for 10 to 30 min. Scatchard plot of (3H)-5-HT binding using a Millpore filter and a Glass filter drew biphasic and monophasic, respectively. It is suggested that filter may affect on specific (3H)-5-HT binding. Therefore, the characteristics of filter should be taken into consideration in (3H)-5-HT binding assay.
About This Article
Keywords
5-HT receptor binding assay
Filter
Low-affinity
High-affinity
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