One hundred and thirty-eight patients with chronic urticaria were tested intradermally with Dermatophagodes pteronyssinus and Dermatophagoides farinae allergens. None had any past history of other atopic diseases. The percentage of cases which gave positive skin reactions to these mite allergens was larger in chronic urticarial patients than in normal healthy subjects. A significant correlation was observed between the magnitude of skin reactions to D. pteronyssinus and to D. farinae. The results suggest that D. pteronyssinus and D. farinae may play a role in chronic urticaria and that there may be cross allergenicity between D. pteronyssinus and D. farinae.