コイ血漿アルブミンの季節的変動

同一養魚池で飼育したマゴイから年4回採血し,血漿成分の季節的変動を測定した。血漿タンパク質は,50％,飽和硫酸アンモニウム溶液に対する溶解度の差を利用して,アルブミンおよびグロブリン画分に分離し,両者の電気泳動的挙動を調べた。

セルロースアセテート電気泳動像に現われたタンパク質成分をアルブミンおよびグロブリン画分にわけ,両者の比の季節的変動をみると,暖期にアルブミン画分が多く,寒冷期に少ない。また,アルブミン性タンパク質のうち脂質を含むアルブミンの動向をディスク電気泳動で観察すると,暖期に多く,寒冷期に少なく,その変動は血漿タンパク質の増減をも支配する。しかしながら,血漿総脂質量と脂質を含むアルブミン量の季節的変動の傾向とは一致しない。

Two-year old carp, Cyprinus carpio, were kept in an out-door pond between May of 1975 and January of 1976. Blood samples were collected at the end of every seasons by cardiac puncture with a heparinized cyringe. The seasonal variation of hematocrit value, plasma total lipid, and plasma sugar levels were measured.

The main 6 fractions were separated from the plasma protein by cellulose acetate electrophoresis. The localization of albumin and globulin fractions on the cellulose acetate electrophoretic diagram was estimated by the preliminary fractionation of the plasma protein by a 50% saturated ammonium sulfate solution. The cellulose acetate electrophoretic analysis showed that the plasma total albumin content was higher in the warm than the cold season.

The main albumin component in red carp has been identified as a kind of lipoprotein in a previous study. However now, in this study, the lipid-containing albumin was separated into 2 components in a disc electrophoresis. The amount of lipid-containing albumins was high in the warm season. The total protein content was also high in the same season. Therefore the fluctuation of carp plasma albumin seems to be connected to the seasonal factor. It was suggested that the variation of lipid-containing albumin had an influence on the plasma total protein level. However, no correlation was observed between the variations of the lipid-containing albumin and plasma total lipid.