To clarify the infection prevention measure taken in NICU, we implemented a questionnaire on 223 NICU facilities which are registered in Japanese Neonatologist Association, and retrieved from 112 facilities (collection rate 50.2%). Hand washing was performed in all facilities when bathing or blanket bathing the patient. 66 facilities (86%) answered that their way of hand washing is "washing hands using soaps or sterilizing reagents followed by spray type disinfectant". On question about whether gloves are used during blanket bathing, 89 facilities (79%) answered yes, and 82 (92%) were using non-sterilized gloves. 106 facilities (94%) cleans incubator every day, where 73 (65%) using sterilizing reagents, 38 (35%) using non-sterilized towels which is the most common way of practice. About the rate of changing incubators, 62 facilities (55%) answered "once a week", and is the most obtained answer. Criterion on changing incubator differs by facilities in terms of the purpose and the points to keep attention. While hand washing is performed as standard precaution in most facilities, criteria and procedure concerning other hygiene care differed. Although many infection prevention measure considering neonatal specificity have unclear clinical evidence, under current situation, it is required to review the effective infection control measure in daily hygiene care with recognition of the facility difference.