An experiment with 12 lactating cows in a 3 x 3 Latin square design determined effects of diets containing whole cottonseed, commercially available heat-flaked soybeans, or these two ingredients on milk production and composition, and total digestible nutrients (TDN) intake. Diet composition was 65% concentrate, 25% Italian ryegrass silage, and 10% alfalfa haycube on TDN basis. Concentrate contained either 30% whole cottonseed, 30% heat-flaked soybeans, or 15% whole cottonseed plus 15% heat-flaked soybeans. All diets were approximately isocaloric and isonitrogenous. The air temparature and relative humidity averaged 28.5°C, ranged from 20.0°C to 36.8°C, and 71.8%. The TDN ratio of intake to requirement was highest for the 30% whole cottonseed, but there were no differences among diets for milk yield, solids not-fat %, or protein % of milk. Fat-corrected milk production for the 30% whole cottonseed tended to be higher than those of the other two diets because of the highest milk fat % (p <0.05).