Thermodynamical and morphological studies were conducted to know the effect of various conc. of Hg2+ on high Hg2+ tolerant Pseudomonas strains (No. 10 & 19) isolated from sewage. In the thermodynamical studies, the lag phase was shortened and the minimum activation energy for growth of these strains (No. 10 & 19) was recognized under 12.5 μg/ml Hg2+ conc.: 2.5 Kcal/mol and 5.0 Kcal/mol respectively. On the other hand, morphological studies revealed that the native cell maintained the shape when Hg2+. conc. was 12.5 μg/ml but in the absence of Hg2+ it was observed to abnormal long shape. The result suggested that 12.5 μg/ml Hg2+ conc. was required for the growth and maintenance of native cell in high Hg2+ tolerant Pseudomonas.