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広島大学生物生産学部紀要 26 巻 1・2 号
1987-11 発行
Effects of Treatments with Human Chorionic Gonadotropin after Successive Administrations of Porcine Follicular Fluid on the Ovulation Rate and Plasma Concentration of Progesterone in Rabbits
活性炭処理豚卵胞液投与後の時間経過が, 家兎の排卵数及びプロジェステロン濃度に及ぼす影響
Nagahama Shinobu
Fukunari Atsushi
Maniwa Jiro
Alfenso Noel F.
Tsutsumi Yoshio
Tozuka Ayako
Sato Kunitada
Abstract
活性炭で処理した豚卵胞液(CTPFF)を家兎に授与し、投与終了時より時間を追ってhCGによる排卵の誘起を試み、それが家兎の排卵数および血漿プロジェステロン濃度に与える影響について検討した
1. CTPFFを4mℓずづ12時間おきに10回連続皮下投与し、その直後に開腹して卵巣表面を観察すると、大型卵胞(≥2.Omm直径)が全く存在しなかったが、対照の生理的食塩水投与区には多数存在していた。
2. 前項1と同様にCTPFFを投与し、投与終了直後(0F-区)、12時間後(12-区)、24時間後(24-区)、36時間後(36-区)、48時間後(48-区)、72時間後(72-区)、96時間後(96-区)の各期にhCG15IUを投与し、4日目に開腹して卵巣表面を観察した。0F-区の排卵数(2.3個)はその他の処理区(6.7～12.0個)及び0S-区(10.2個)と比較して有意に少なかった。平均排卵数は12-区(6.7個)で0S-区(10.2個)より有意に少なかったのに対し、36-区(12.0個)で最も多く(最高18個)、その他の区ではほとんどかわらなかった。また、12-区には大型卵胞(1.5mm≥2.0mm直径)が多く存在していた。末梢血漿中のプロジェステロン濃度(RIA法)はCTPFF処理中は対照区よりも僅かに高かった。12-区はhCG投与後からプロジェステロン濃度が高くなったが、その他の区ではhCG投与後に一度低下し、以後増加した。また、24～72-区はhCG投与4時間後に高い値を示した。外陰部の腫脹・潮紅の度合にはCTPFF投与翌日から低下し、投与終了後に少し回復した。
以上の結果、CTPFFの連続投与が家兎の排卵に対する抑制的影響は比較的早く失われ、CTPFF投与終了後36時間までにFSH濃度にリバウンドが起きていることが推測された。また、プロジェステロン濃度は処理区で高く、ステロイドの生合成に何らかの変化が起きているものと推測された。
1. CTPFFを4mℓずづ12時間おきに10回連続皮下投与し、その直後に開腹して卵巣表面を観察すると、大型卵胞(≥2.Omm直径)が全く存在しなかったが、対照の生理的食塩水投与区には多数存在していた。
2. 前項1と同様にCTPFFを投与し、投与終了直後(0F-区)、12時間後(12-区)、24時間後(24-区)、36時間後(36-区)、48時間後(48-区)、72時間後(72-区)、96時間後(96-区)の各期にhCG15IUを投与し、4日目に開腹して卵巣表面を観察した。0F-区の排卵数(2.3個)はその他の処理区(6.7～12.0個)及び0S-区(10.2個)と比較して有意に少なかった。平均排卵数は12-区(6.7個)で0S-区(10.2個)より有意に少なかったのに対し、36-区(12.0個)で最も多く(最高18個)、その他の区ではほとんどかわらなかった。また、12-区には大型卵胞(1.5mm≥2.0mm直径)が多く存在していた。末梢血漿中のプロジェステロン濃度(RIA法)はCTPFF処理中は対照区よりも僅かに高かった。12-区はhCG投与後からプロジェステロン濃度が高くなったが、その他の区ではhCG投与後に一度低下し、以後増加した。また、24～72-区はhCG投与4時間後に高い値を示した。外陰部の腫脹・潮紅の度合にはCTPFF投与翌日から低下し、投与終了後に少し回復した。
以上の結果、CTPFFの連続投与が家兎の排卵に対する抑制的影響は比較的早く失われ、CTPFF投与終了後36時間までにFSH濃度にリバウンドが起きていることが推測された。また、プロジェステロン濃度は処理区で高く、ステロイドの生合成に何らかの変化が起きているものと推測された。
Abstract
Effects of treatments for induced ovulation with hCG during 96 hours after successive administrations of charcoal-treated porcine follicular fluid (CTPFF) on the ovulation rate and plasma concentration of progesterone (P) in rabbits were examined.
In experiment 1, four does or three does which were injected with 4 ml of CTPFF or saline (control), respectively, at 12-hour intervals for 5 days (10 times) showed no difference in the total number of follices. No large follicles (≥ 2.0 mm in diameter) appeared in ovaries of the treated group, in comparison with their presence in those of the control.
In experiment 2, forty-two does were injected with 4 ml of CTPFF in the same manner as in experiment 1. Thirty-six of them were divided into 6 groups according to the number of hours from the final administration of CTPFF to hCG injection-12, 24, 36, 48, 72 and 96 hours. The number of ovulations in six other does which were ovulated by injection of hCG simultaneously with the final administration of CTPFF (group 0F) were significantly lower than those of any other group, and the number increased with interval up to 36 hours from the end of CTPFF treatment to hCG injection. The number of follicles of large size (≥ 2.0 mm in diamter) in ovaries of does in group 12 (12-hour interval) at 4 days after hCG injection was larger than in any other treatment group.
In does of groups 24, 36, 48 and 72, plasma P levels 4 hours after hCG injection were higher than those of the other groups of does. In group 12, P levels at 4 days after hCG injection were higher than those of any other group.
Vulval swelling and coloration receded 1 day after the beginning of CTPFF treatment, and recovered slightly after the end of CTPFF treatment until hCG injection. These results indicate that successive treatments of CTPFF for 5 days during the follicular phase decrease ovulation rate, and the inhibitory effect on ovulation is lost within a short time. Follicle-stimulating hormone levels may rebound within 36 hours after the final administration of CTPFF treatment, because the ovulation rate in group 36 was the highest.
In experiment 1, four does or three does which were injected with 4 ml of CTPFF or saline (control), respectively, at 12-hour intervals for 5 days (10 times) showed no difference in the total number of follices. No large follicles (≥ 2.0 mm in diameter) appeared in ovaries of the treated group, in comparison with their presence in those of the control.
In experiment 2, forty-two does were injected with 4 ml of CTPFF in the same manner as in experiment 1. Thirty-six of them were divided into 6 groups according to the number of hours from the final administration of CTPFF to hCG injection-12, 24, 36, 48, 72 and 96 hours. The number of ovulations in six other does which were ovulated by injection of hCG simultaneously with the final administration of CTPFF (group 0F) were significantly lower than those of any other group, and the number increased with interval up to 36 hours from the end of CTPFF treatment to hCG injection. The number of follicles of large size (≥ 2.0 mm in diamter) in ovaries of does in group 12 (12-hour interval) at 4 days after hCG injection was larger than in any other treatment group.
In does of groups 24, 36, 48 and 72, plasma P levels 4 hours after hCG injection were higher than those of the other groups of does. In group 12, P levels at 4 days after hCG injection were higher than those of any other group.
Vulval swelling and coloration receded 1 day after the beginning of CTPFF treatment, and recovered slightly after the end of CTPFF treatment until hCG injection. These results indicate that successive treatments of CTPFF for 5 days during the follicular phase decrease ovulation rate, and the inhibitory effect on ovulation is lost within a short time. Follicle-stimulating hormone levels may rebound within 36 hours after the final administration of CTPFF treatment, because the ovulation rate in group 36 was the highest.
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