The Higashi-Hiroshima Campus, Hiroshima University, is located in the center of Higashi-Hiroshima City, Hiroshima Prefecture. After the campus was relocated from Hiroshima City, two surveys of Avian fauna were conducted, from May 1998 to April 1999 and April 2010 to March 2011. At Hiroshima University Museum, a campus rich in nature is regarded as a “Whole Campus Museum,” and biota surveys have been conducted. We investigated the effect of environmental changes after relocation on the avian fauna as the previous surveys were done a long time back. Using the route-census method, as in the previous surveys, we conducted the survey once a week for two years, from April 2018 to March 2020. As a result, in 2018, we confirmed 6,854 wild birds of 13 orders, 29 families, and 68 species. In 2019, we confirmed 13,368 wild birds of 14 orders, 31 families, and 68 species. Previous studies had reported that this research site serves as a green corridor and a staging area for migration. This study further revealed that this research site is used as a breeding ground for about 10 species, including Narcissus flycatcher (Ficedula narcissina). Furthermore, after the campus relocation, it became clear that the avian fauna and species that emerged due to the succession of vegetation have changed. Hiroshima Prefecture was hit by heavy rains in western Japan in July 2018, and 2020 had a very warm winter. Therefore, extreme weather was also thought to affect the appearance of wild birds.