The purpose of this study was to examine the effect of repeated listening of musical pieces on musical impressions, emotional responses and on heart rate (HR). Twelve subjects rated musical impressions and emotional responses, and were measured HR under the three experimental conditions; sedative music, excitative music, and no music conditions. Subjects received four times of each experimental condition sequentially. The results were as follows. 1) At the first listening of music, subjects' ratings on musical impression of activity and potency reflected the characteristics of musical pieces. Those impressions were not change by repeated listening of music. 2) Sedative music promoted the emotions, 'relaxed' and 'fascinated' at the first listening and those effects were maintained through the experimental session. On the other hand excitative music restrained the emotions, 'relaxed' and 'fascinated' at the first listening and promoted the emotion, 'aggressive'. Those effects of excitative music diminished by repeated listening. 3) There were the effects that excitative music blocked HR decrease and that sedative music facilitated HR decrease. 4) There was no intra-individual stability on the HR pattern during music listening.