Branched-chain amino acids-induced cardiac protection against ischemia/reperfusion injury

Life Sciences Volume 245 Page 117368- published_at 2020-03-15
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Title ( eng )
Branched-chain amino acids-induced cardiac protection against ischemia/reperfusion injury
Creator
Satomi Shiho
Morio Atsushi
Miyoshi Hirotsugu
Tsutsumi Rie
Sakaue Hiroshi
Source Title
Life Sciences
Volume 245
Start Page 117368
Abstract
Aims: Amino acids, especially branched chain amino acids (BCAAs), have important regulatory roles in protein synthesis. Recently studies revealed that BCAAs protect against ischemia/reperfusion (I/R) injury. We studied the signaling pathway and mitochondrial function affecting a cardiac preconditioning of BCAAs.
Main methods: An in vivo model of I/R injury was tested in control, mTOR+/+, and mTOR+/−. Mice were randomly assigned to receive BCAAs, rapamycin, or BCAAs + rapamycin. Furthermore, isolated cardiomyocytes were subjected to simulated ischemia and cell death was quantified. Biochemical and mitochondrial swelling assays were also performed.
Key findings: Mice treated with BCAAs had a significant reduction in infarct size as a percentage of the area at risk compared to controls (34.1 ± 3.9% vs. 44.7 ± 2.6%, P = 0.001), whereas mice treated with the mTOR inhibitor rapamycin were not protected by BCAA administration (42.2 ± 6.5%, vs. control, P = 0.015). This protection was not detected in our hetero knockout mice of mTOR. Western blot analysis revealed no change in AKT signaling whereas activation of mTOR was identified. Furthermore, BCAAs prevented swelling which was reversed by the addition of rapamycin. In myocytes undergoing simulated I/R, BCAA treatment significantly preserved cell viability (71.7 ± 2.7% vs. 34.5 ± 1.6%, respectively, p < 0.0001), whereas rapamycin prevented this BCAA-induced cardioprotective effect (43.5 ± 3.4% vs. BCAA, p < 0.0001).
Significance: BCAA treatment exhibits a protective effect in myocardial I/R injury and that mTOR plays an important role in this preconditioning effect.
Keywords
Amino acid
Ischemia
Reperfusion
mTOR
Mitochondria
Descriptions
This work was supported by JSPS KAKENHI, Japan [grant number 19K09353].
Language
eng
Resource Type journal article
Publisher
Elsevier
Date of Issued 2020-03-15
Rights
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 0024-3205
[DOI] 10.1016/j.lfs.2020.117368
[PMID] 32001270
[DOI] https://doi.org/10.1016/j.lfs.2020.117368
Remark Post-print version/PDF may be used in an institutional repository after an embargo period of 12 months.