Quantitative Determination of Arachidonate 5-Lipoxygenase Metabolites in Human Polymorphonuclear Leukocytes by Reverse-Phase High-Performance Liquid Chromatography
Hiroshima Journal of Medical Sciences 35 巻 2 号
99-103 頁
1986-06 発行
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この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00049907
| ファイル情報(添付) | |
| タイトル ( eng ) |
Quantitative Determination of Arachidonate 5-Lipoxygenase Metabolites in Human Polymorphonuclear Leukocytes by Reverse-Phase High-Performance Liquid Chromatography
|
| 作成者 |
HAMASAKI Takashi
|
| 収録物名 |
Hiroshima Journal of Medical Sciences
|
| 巻 | 35 |
| 号 | 2 |
| 開始ページ | 99 |
| 終了ページ | 103 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
Quantitative analysis system of leukotriene B4, C4, D4 (LTB4, LTC4, LTD4), and 5-hydroxyeicosatetraenoic acid (5-HETE) by reverse-phase high-performance liquid chromatography was developed. The lower limit of detection was 1 ng of each compound with a very close correlation between the amount of each compound and its peak height. Normal peripheral blood polymorphonuclear leukocytes (PMN) stimulated with Ca ionophore A23187, produced detectable amounts of LTB4, LTC4, and 5-HETE, but no LTD4 was produced in this condition. This analysis system was useful for measuring LTB4, LTC4, LTD4, and HETEs from the biological samples.
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| 著者キーワード |
Leukotrienes
HETEs
High performance liquid chromatography
|
| NDC分類 |
医学 [ 490 ]
|
| 言語 |
英語
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| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University School of Medicine
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| 発行日 | 1986-06 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3021690
|
