Quantitative Determination of Arachidonate 5-Lipoxygenase Metabolites in Human Polymorphonuclear Leukocytes by Reverse-Phase High-Performance Liquid Chromatography
Hiroshima Journal of Medical Sciences Volume 35 Issue 2
Page 99-103
published_at 1986-06
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| File | |
| Title ( eng ) |
Quantitative Determination of Arachidonate 5-Lipoxygenase Metabolites in Human Polymorphonuclear Leukocytes by Reverse-Phase High-Performance Liquid Chromatography
|
| Creator |
HAMASAKI Takashi
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 35 |
| Issue | 2 |
| Start Page | 99 |
| End Page | 103 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Quantitative analysis system of leukotriene B4, C4, D4 (LTB4, LTC4, LTD4), and 5-hydroxyeicosatetraenoic acid (5-HETE) by reverse-phase high-performance liquid chromatography was developed. The lower limit of detection was 1 ng of each compound with a very close correlation between the amount of each compound and its peak height. Normal peripheral blood polymorphonuclear leukocytes (PMN) stimulated with Ca ionophore A23187, produced detectable amounts of LTB4, LTC4, and 5-HETE, but no LTD4 was produced in this condition. This analysis system was useful for measuring LTB4, LTC4, LTD4, and HETEs from the biological samples.
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| Keywords |
Leukotrienes
HETEs
High performance liquid chromatography
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1986-06 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3021690
|
