Albumin permeability across endothelial cell monolayer exposed to reactive oxygen intermediates : Involvement of reversible functional alteration of the cell membrane Ca2+channels

アクセス数 : 878
ダウンロード数 : 98

今月のアクセス数 : 1
今月のダウンロード数 : 1
File
diss_ko2110.pdf 690 KB 種類 : fulltext
Title ( eng )
Albumin permeability across endothelial cell monolayer exposed to reactive oxygen intermediates : Involvement of reversible functional alteration of the cell membrane Ca2+channels
Title ( jpn )
活性酸素種曝露による血管内皮細胞単層膜のアルブミン透過性 : 細胞膜カルシウムチャネルの可逆的な機能変化
Creator
Abstract
This study was designed to test the idea that the redox state of sulfhydryl (SH)-groups in cell-membrane Ca2+ channels plays a pivotal role in Ca2+in flux, which in turn causes an increase in albumin permeability across the cultured monolayer of porcine pulmonary artery endothelial (PPAE) cells exposed to xanthine/xanthine oxidase (X/XO). Albumin permeability as well as the concentration of intracellular Ca2+( [Ca2+]i) was increased by XKO. A Hz02 scavenger (catalase), an iron chelator (o-phenanthroline), and a hydroxyl radical scavenger (dimethyl sulfoxide) inhibited these changes provoked by XKO, in which intracellular iron-catalyzed hydroxyl radical generation was suggested to be involved. The increase in albumin permeability and [Ca2+]i continued once the PPAE cells were exposed to XKO. The [Ca2+]wi as decreased by a Ca2+ channel blocker, Ni2+, while the removal of Ni2+in creased [Ca2+]I again, suggesting the sustained Ca2+ influx through cell-membrane Ca" channels was responsible for the [Ca2+]i elevation. Ni2+f ailed to inhibit albumin permeability sustained after the removal of XKO. In contrast, SH-reducing agents (dithiothreitol and glutathione) inhibited the sustained permeability as well as Ca2+ influx. We concluded that the redox alteration of SH-groups in cell-membrane Ca2+ channels was involved in the increase in albumin permeability after exposure of the endothelial cells to oxidative stress.
Keywords
Albumin permeability
Reactive oxygen intermediates
Calcium channels
Redox state
Descriptions
Hiroshima J. Med. Sci. Vol.49, No.1, 57-65, March, 2000 HIJM49-8
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type doctoral thesis
Rights
Copyright (c) by Author
Access Rights open access
Dissertation Number 甲第2110号
Degree Name
Date of Granted 2000-03-07
Degree Grantors
広島大学