A total of 41 leptocephali was collected in the Kuroshio waters off Shikoku Island. They included 33 Gnathophis nystromi nystromi (JORDAN et SNYDER), one Ariosoma sp. 5, two Ariosoma sp. 7, one Conger japonicus BLEEKER, two Congrinae sp. 1, and two Dysomma anguillare BARNARD. Most of leptocephali collected (82.9% of a total catch) were occurred in the southern boundary of the Kuroshio Current. All the Gnathophis leptocephali were collected with the surface horizontal tow of High Speed Larva Net, and the other leptocephali were with step tow of Larva Net.