Since the mid-twentieth century, higher education systems worldwide went through huge transformations as a consequence of massification and the recognition of knowledge as key for economic development. The emergence of new teaching modes, the intensity of research, and performativity and accountability pressures impacted directly on academic work and, as a result, on academic working conditions. In this article, we examine the changes that the Argentine professoriate has experienced in recent decades. We argue that the process of fragmentation of the academic profession presents particularities in regard to global trends. We analyze the current state and evolution of the academic workforce in public universities by focusing on different aspects related to its academic profile: gender, training, working conditions, employers, positions, types of contracts, academic practice, perceptions about the career, preferences, and job stress/satisfaction. To achieve this, we have relied on data from the Academic Profession in the Knowledge-Based Society (APIKS) survey.