We conducted underwater census to survey the fish fauna on reefs of Tokara Islands with aids of the research vessel Toyoshio-maru of Hiroshima University. A total 304 fish species were recorded, and were almost completely comprised of coral reef fishes mainly distributing in the tropical or subtropical waters. This result strongly suggests that Tokara Islands biogeographically belong to the subtropical-water area as a part of the Ryukyu Islands district as ever been proposed by Nakabo (2002a). Through comparisons of the present fish records with data of further northern areas in Kuroshio Current zone, a total of 11 species, Neoniphon opercularis (Holocentridae), Pseudochromis marshallensis (Pseudochromidae), Apogon exostigma (Apogonidae), Hoplolatilus cuniculus (Malacanthidae), Amphiprion frenatus (Pomacentridae), Cheilinus fasciatus (Labridae), Chlorurus oedema (Scaridae), Ecsenius oculus (Blenniidae), Fusigobius humeralis (Gobiidae), Siganus guttatus (Siganidae), Naso tuberosus (Acanthuridae) are suggested to distribute up to Tokara Islands as northern range limits.