観察教材としてのミズカビの培養法の改良

学校教育実践学研究 Volume 23 Page 27-30 published_at 2017-03-22
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Title ( jpn )
観察教材としてのミズカビの培養法の改良
Title ( eng )
Modification of Culturing Method for Saprolegnia sp. as the Observation Material in Biology
Creator
Takao Akira
Source Title
学校教育実践学研究
Hiroshima journal of school education
Volume 23
Start Page 27
End Page 30
Journal Identifire
[ISSN] 1341-111X
[NCID] AN10491493
Abstract
Saprolegnia spp., common aquatic fungi, are known as one of the appropriate material to observe the reproductive process in its life cycle. The aim of the present study is to modify culturing method of Saprolegnia sp. We prepared culture media containing various concentration of the Cannabis extract solidified by agar. The hyphae of Saprolegnia sp. were cultured in sterilized water with small pieces of the agar media. As the result, many zoosporangia and archegonia were observed under the light microscope. In comparison with the ordinary culturing method using whole cotyledon of Cannabis, the colonies of Saprolegnia sp. obtained by this modified method were suitable to observe various stage of life cycle. The nutritional conditions in culturing are able to be controlled by this method. Therefore, it will be used as the practical method in the biology class.
Keywords
Saprolegnia
life cycle
culturing method
Descriptions
本研究は,科学研究費基盤研究(A)(一般)(25242015)および科学研究費基盤研究(C)(一般)(26350235)の助成を受けて行った。
NDC
Education [ 370 ]
Language
jpn
Resource Type departmental bulletin paper
Publisher
広島大学大学院教育学研究科附属教育実践総合センター
Date of Issued 2017-03-22
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 1341-111X
[NCID] AN10491493