Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves
Biological and Pharmaceutical Bulletin 33 巻 10 号
1673-1679 頁
2010-10-01 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves
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| 作成者 |
Tamura Takayoshi
Ozaki Moeko
Maruyama Masafumi
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| 収録物名 |
Biological and Pharmaceutical Bulletin
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| 巻 | 33 |
| 号 | 10 |
| 開始ページ | 1673 |
| 終了ページ | 1679 |
| 抄録 |
In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7±6.4 g/l GABA at a conversion rate of 86.0±5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG.
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| 著者キーワード |
plant-derived lactic acid bacteria
Enterococcus avium
gamma-aminobutyric acid
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| 内容記述 |
This work was supported by Hiroshima Biocluster (a Cooperative Link of Unique Science and Technology for Economy Revitalization), Japan (M.S.).
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| NDC分類 |
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
The Pharmaceutical Society of Japan
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| 発行日 | 2010-10-01 |
| 権利情報 |
© 2010 Pharmaceutical Society of Japan
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0918-6158
[ISSN] 1347-5215
[NCID] AA10885497
[DOI] 10.1248/bpb.33.1673
[DOI] https://doi.org/10.1248/bpb.33.1673
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