Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves
Biological and Pharmaceutical Bulletin Volume 33 Issue 10
Page 1673-1679
published_at 2010-10-01
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Title ( eng ) |
Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves
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Creator |
Tamura Takayoshi
Ozaki Moeko
Maruyama Masafumi
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Source Title |
Biological and Pharmaceutical Bulletin
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Volume | 33 |
Issue | 10 |
Start Page | 1673 |
End Page | 1679 |
Abstract |
In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7±6.4 g/l GABA at a conversion rate of 86.0±5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG.
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Keywords |
plant-derived lactic acid bacteria
Enterococcus avium
gamma-aminobutyric acid
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Descriptions |
This work was supported by Hiroshima Biocluster (a Cooperative Link of Unique Science and Technology for Economy Revitalization), Japan (M.S.).
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | journal article |
Publisher |
The Pharmaceutical Society of Japan
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Date of Issued | 2010-10-01 |
Rights |
© 2010 Pharmaceutical Society of Japan
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Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0918-6158
[ISSN] 1347-5215
[NCID] AA10885497
[DOI] 10.1248/bpb.33.1673
[DOI] https://doi.org/10.1248/bpb.33.1673
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