High and stable substrate specificities of microorganisms in enhanced biological phosphorus removal plants

Environmental Microbiology Volume 15 Issue 6 Page 1821-1831 published_at 2013-06
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Title ( eng )
High and stable substrate specificities of microorganisms in enhanced biological phosphorus removal plants
Creator
Nierychlo Marta
Kragelund Caroline
Nielsen Jeppe Lund
Nielsen Per Halkjær
Source Title
Environmental Microbiology
Volume 15
Issue 6
Start Page 1821
End Page 1831
Abstract
Microbial communities are typically characterized by conditions of nutrient limitation so the availability of the resources is likely a key factor in the niche differentiation across all species and in the regulation of the community structure. In this study we have investigated whether four species exhibit any in situ short-term changes in substrate uptake pattern when exposed to variations in substrate and growth conditions. Microautoradiography was combined with fluorescence in situ hybridization to investigate in situ cell specific substrate uptake profiles of four probe-defined coexisting species in a wastewater treatment plant with enhanced biological phosphorus removal. These were the filamentous “Candidatus Microthrix” and Caldilinea (type 0803), the polyphosphate-accumulating organism “Candidatus Accumulibacter”, and the denitrifying Azoarcus. The experimental conditions mimicked the conditions potentially encountered in the respective environment (starvation, high/low substrate concentration, induction with specific substrates, and single/multiple substrates). The results showed that each probe-defined species exhibited very distinct and constant substrate uptake profile in time and space, which hardly changed under any of the conditions tested. Such niche partitioning implies that a significant change in substrate composition will be reflected in a changed community structure rather than the substrate uptake response from the different species.
Descriptions
This research was partially supported by the JSPS Institutional Program for Young Researcher Overseas Visits (T. Kindaichi), by research foundation from Public Association for Construction Service Chugoku region (T. Kindaichi), by FøSu (M. Nierychlo and P.H. Nielsen), and by Aalborg University.
This is the peer reviewed version of the following article: High and stable substrate specificities of microorganisms in enhanced biological phosphorus removal plants, Environmental Microbiology, 15(6), 1821-1831, which has been published in final form at [DOI: 10.1111/1462-2920.12074]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
NDC
Construction. Civil engineering [ 510 ]
Language
eng
Resource Type journal article
Publisher
Wiley
Date of Issued 2013-06
Rights
Copyright (c) 2012 John Wiley & Sons Ltd and Society for Applied Microbiology
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 1462-2912
[DOI] 10.1111/1462-2920.12074
[DOI] http://doi.org/10.1111/1462-2920.12074 isVersionOf