簡易蛍光顕微鏡の開発と高等学校生物における活用の提案

学校教育実践学研究 Volume 22 Page 143-148 published_at 2016-03-22
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Title ( jpn )
簡易蛍光顕微鏡の開発と高等学校生物における活用の提案
Title ( eng )
Development of a simple, hand made fluorescence microscope and a proposal to its practical use for the high school biology
Creator
Saiga Daisuke
Maga Ayane
Kinutani Kazuko
Fukuda Mika
Source Title
学校教育実践学研究
Hiroshima journal of school education
Volume 22
Start Page 143
End Page 148
Journal Identifire
[ISSN] 1341-111X
[NCID] AN10491493
Abstract
In the present study, a simple hand made fluorescence microscope was developed. Four UV-LED lamps (NS360L-5RLO, Nitride Semiconductors Co., Ltd.) were used for the ultraviolet irradiation device. Some problems of a sample device have been improved and that has been completed. As the result, the nuclear fluorescence of the epidermal tissue of Tradescantia ohiensis stained by DAPI (4',6-diamidino-2-phenylindole) were observed.

This low cost simple fluorescence microscope was very effective for the student’s activities in the high school biology classes. In this paper, authors proposed an example of experiment which analyze relative DNA content value of nucleus in mitotic cell cycle by using this fluorescence microscope.
Descriptions
本研究の一部は,科学研究費基盤研究A(25242015)および科学研究費基盤研究C(26350235)の助成を受けて行ったものである。
NDC
Education [ 370 ]
Language
jpn
Resource Type departmental bulletin paper
Publisher
広島大学大学院教育学研究科附属教育実践総合センター
Date of Issued 2016-03-22
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 1341-111X
[NCID] AN10491493