Differential gene regulation by VIV and VV ions in the branchial sac, intestine, and blood cells of a vanadium-rich ascidian, Ciona intestinalis
BioMetals 25 巻 5 号
1037-1050 頁
2012-10 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Differential gene regulation by VIV and VV ions in the branchial sac, intestine, and blood cells of a vanadium-rich ascidian, Ciona intestinalis
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| 作成者 |
Kume Satoshi
Matsuoka Hiroki
Hamada Mayuko
Satoh Nori
Michibata Hitoshi
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| 収録物名 |
BioMetals
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| 巻 | 25 |
| 号 | 5 |
| 開始ページ | 1037 |
| 終了ページ | 1050 |
| 抄録 |
Ascidians are hyperaccumulators that have been studied in detail. Proteins and genes involved in the accumulation process have been identified, but regulation of gene expression related to vanadium accumulation remains unknown. To gain insights into the regulation of gene expression by vanadium in a genome-wide manner, we performed a comprehensive study on the effect of excess vanadium ions on a vanadium-rich ascidian, Ciona intestinalis, using a microarray. RT-PCR and enzyme activity assay were performed from the perspective of redox and accumulation of metal ions in each tissue. Glutathione metabolism-related proteins were significantly up-regulated by VIV treatment. Several genes involved in the transport of vanadium and protons, such as Nramp and V-ATPase, were significantly up-regulated by VIV treatment. We observed significant up-regulation of glutathione synthesis and degradation pathways in the intestine and branchial sac. In blood cells, expression of Ci-Vanabin4, glutathione reductase activity, glutathione levels, and vanadium concentration increased after VIV treatment. VIV treatment induced significant changes related to vanadium exclusion, seclusion, and redox pathways in the intestine and branchial sac. It also induced an enhancement of the vanadium reduction and accumulation cascade in blood cells. These differential responses in each tissue in the presence of excess vanadium ions suggest that vanadium accumulation and reduction may have regulatory functions. This is the first report on the gene regulation by the treatment of vanadium-rich ascidians with excess vanadium ions. It provided much information for the mechanism of regulation of gene expression related to vanadium accumulation.
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| 著者キーワード |
Ascidian
Vanadium
Microarray
Gene regulation
Glutathione
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| 内容記述 |
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (#21570077 to H. M. and #20570070 to T. U.) and a grant from the Toray Science Foundation (03-4402 to T. U.).
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| NDC分類 |
生物科学・一般生物学 [ 460 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Springer Netherlands
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| 発行日 | 2012-10 |
| 権利情報 |
Copyright (c) Springer Science+Business Media, LLC. 2012
The final publication is available at Springer via http://dx.doi.org/10.1007/s10534-012-9569-z.
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0966-0844
[NCID] AA10834000
[DOI] 10.1007/s10534-012-9569-z
[DOI] http://doi.org/10.1007/s10534-012-9569-z
～の異版である
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