HISTAMINASE AND ITS INHIBITORY FACTOR IN GUINEA PIG SKIN

Hiroshima Journal of Medical Sciences Volume 30 Issue 3 Page 251-254 published_at 1981-09-10
アクセス数 : 744
ダウンロード数 : 101

今月のアクセス数 : 0
今月のダウンロード数 : 0
File
HiroshimaJMedSci_30_251.pdf 2.62 MB 種類 : fulltext
Title ( eng )
HISTAMINASE AND ITS INHIBITORY FACTOR IN GUINEA PIG SKIN
Title ( jpn )
モルモット皮膚のヒスタミナーゼとその阻害因子
Creator
Yamamoto Shoso
Source Title
Hiroshima Journal of Medical Sciences
Volume 30
Issue 3
Start Page 251
End Page 254
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
The crude histaminase preparation obtained from guinea pig skin was fractionated by Sephadex G-200 at 4°C and 55°C. The histaminase activity corresponded to the descending limb of the first peak of protein eluted just after void volume at 4°C and to the second peak at 55°C. The histaminase activity in the fractions eluted at 4°C was higher at 55°C than at 37°C. On the contrary, no significant difference between the histaminase activity assayed at 55°C and 37°C was observed in the fractions eluted at 55°C. On Sephadex G-200 gel filtration, histaminase was eluted in the later fractions at 55°C, compared with the fractions eluted at 4°C, indicating that the molecular weight of histaminase may become smaller at 55°C. These facts suggest that histaminase obtained from guniea pig skin may bind with an inhibitor-like substance at low temperature and that the enzyme may separate from the inhibitor at high temperature.
Descriptions
This work was supported in part by Scientific Research Grant from the Japanese Ministry of Education (C, No. 56570383).
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University School of Medicine
Date of Issued 1981-09-10
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6795150