HISTAMINASE AND ITS INHIBITORY FACTOR IN GUINEA PIG SKIN

モルモット皮膚のヒスタミナーゼとその阻害因子

Yamamoto Shoso

Hiroshima Journal of Medical Sciences

[PISSN] 0018-2052

[EISSN] 2433-7668

[NCID] AA00664312

The crude histaminase preparation obtained from guinea pig skin was fractionated by Sephadex G-200 at 4°C and 55°C. The histaminase activity corresponded to the descending limb of the first peak of protein eluted just after void volume at 4°C and to the second peak at 55°C. The histaminase activity in the fractions eluted at 4°C was higher at 55°C than at 37°C. On the contrary, no significant difference between the histaminase activity assayed at 55°C and 37°C was observed in the fractions eluted at 55°C. On Sephadex G-200 gel filtration, histaminase was eluted in the later fractions at 55°C, compared with the fractions eluted at 4°C, indicating that the molecular weight of histaminase may become smaller at 55°C. These facts suggest that histaminase obtained from guniea pig skin may bind with an inhibitor-like substance at low temperature and that the enzyme may separate from the inhibitor at high temperature.

Medical sciences [ 490 ]

eng

Hiroshima University School of Medicine

[ISSN] 0018-2052

[NCID] AA00664312

[PMID] 6795150