Attempts at Cultivation of Mycobacterium leprae in Cell Culture under Regulation of Redox Potential at Environment
Hiroshima Journal of Medical Sciences 31 巻 2 号
141-143 頁
1982-06-10 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Attempts at Cultivation of Mycobacterium leprae in Cell Culture under Regulation of Redox Potential at Environment
|
| タイトル ( jpn ) |
酸化還元電位を調整した環境におけるらい菌の細胞培養のこころみ
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| 作成者 |
Matsuo Yoshiyasu
Tatsukawa Hiroshi
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| 収録物名 |
Hiroshima Journal of Medical Sciences
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| 巻 | 31 |
| 号 | 2 |
| 開始ページ | 141 |
| 終了ページ | 143 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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| 抄録 |
Cultivation of Mycobacterium leprae in cell culture was attempted under lower redox potential by adding such reducing agents as reduced glutathione, DL-cysteine hydrochloride, and dithiothreitol to culture medium, and by reduced oxygen tension varying the depth of medium or applying agar suspension technique. During a five years' period since 1976, a slight increase in the number of acid-fast bacilli at harvests was occasionally observed. However, the viability of bacilli recovered was unable to be proven by the mouse foot pad method. It is uncertain whether lower redox potential at environment is strictly requested for cultivation of M. leprae.
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| 著者キーワード |
Mycobacterium leprae
Cell culture
Redox potential
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| 内容記述 |
This work was supported in part by the Japan-US Cooperative Medical Science Program and the Sasakawa Memorial Health Foundation.
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| NDC分類 |
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University School of Medicine
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| 発行日 | 1982-06-10 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6752102
|
