Attempts at Cultivation of Mycobacterium leprae in Cell Culture under Regulation of Redox Potential at Environment
Hiroshima Journal of Medical Sciences Volume 31 Issue 2
Page 141-143
published_at 1982-06-10
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| File | |
| Title ( eng ) |
Attempts at Cultivation of Mycobacterium leprae in Cell Culture under Regulation of Redox Potential at Environment
|
| Title ( jpn ) |
酸化還元電位を調整した環境におけるらい菌の細胞培養のこころみ
|
| Creator |
Matsuo Yoshiyasu
Tatsukawa Hiroshi
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 31 |
| Issue | 2 |
| Start Page | 141 |
| End Page | 143 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Cultivation of Mycobacterium leprae in cell culture was attempted under lower redox potential by adding such reducing agents as reduced glutathione, DL-cysteine hydrochloride, and dithiothreitol to culture medium, and by reduced oxygen tension varying the depth of medium or applying agar suspension technique. During a five years' period since 1976, a slight increase in the number of acid-fast bacilli at harvests was occasionally observed. However, the viability of bacilli recovered was unable to be proven by the mouse foot pad method. It is uncertain whether lower redox potential at environment is strictly requested for cultivation of M. leprae.
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| Keywords |
Mycobacterium leprae
Cell culture
Redox potential
|
| Descriptions |
This work was supported in part by the Japan-US Cooperative Medical Science Program and the Sasakawa Memorial Health Foundation.
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1982-06-10 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6752102
|
