Measurement of Lipase Activity of Guinea Pig Peritoneal Macrophages with 4-Methylumbelliferyl-oleate

4-Methylumbelliferyl-oleate を用いるモルモット腹腔マクロファージのリバーゼ活性測定法

Kiyotani Katsuhiro

Tasaka Hiromichi

Matsuo Yoshiyasu

Hiroshima Journal of Medical Sciences

[PISSN] 0018-2052

[EISSN] 2433-7668

[NCID] AA00664312

Some basic conditions for fluorometric measurement of lipase activity of guinea pig peritoneal macrophages were investigated using 4-methylumbelliferyl-oleate as a substrate. The most adequate condition for the assay is as follows:

i ) Enzyme preparation recommended is the supernatant of ultrasonicate after freezing and thawing of guinea pig peritoneal macrophages suspended in distilled water.

ii) Final concentration of buffered substrate should be adjusted at 0.1 mM in acetate buffer, pH 4.5, free from Triton X-100.

iii) Reaction is terminated by addition of 50 mM Tris-HCl buffer, pH 8.6, instead of 50 mM glycine buffer, pH 10.4.

4-Methylumbelliferyl-oleate

Lipase

Triton X-100

医学 [ 490 ]

英語

Hiroshima University School of Medicine

[ISSN] 0018-2052

[NCID] AA00664312

[PMID] 6688069